谭  镭1，2， 詹  雁2， 阮  佳2， 徐超群2
（1.四川大学华西药学院，四川 成都 610041；2.四川省中医药科学院，四川 成都 610041）
摘  要：建立UPLC测定黄连中盐酸小檗碱和盐酸巴马汀含量的方法。采用ACQUITY UPLC BEH-C18色谱柱（2.1 mm×50 mm×1.7 μm），以乙腈-0.05 mol/L磷酸二氢钾溶液为流动相，梯度洗脱，流速为0.2 mL/min，检测波长为350 nm。结果表明盐酸小檗碱和盐酸巴马汀分别在0.037 2～0.297 6 μg/mL，0.008 2～0.065 6 μg/mL的浓度范围内均有良好的线性关系（r=0.999 9），回收率均大于95%，RSD均小于2%。该方法快速、准确、重现性好，可用于测定黄连中盐酸小檗碱和盐酸巴马汀的含量。
关键词：黄连；盐酸小檗碱；盐酸巴马汀；超高效液相色谱法（UPLC）
中图分类号：S567.5+2；TQ462+.12；R284.1；O657.31     文献标志码：A     文章编号：1674-5124（2013）03-0054-03
UPLC simultaneous determination of berberine hydrochloride and
palmatine hydrochloride in Coptis
TAN Lei1，2， ZHAN Yan2， RUAN Jia2， XU Chao-qun2
（1.West China School of pharmacy，Sichuan University，Chengdu 610041，China；
2.Sichuan Academy of Chinese Medicine Sciences，Chengdu 610041，China）
Abstract: A method was established for simultaneously determining berberine hydrochloride and palmatine hydrochloride in Coptis by UPLC. The optimal conditions of separation and detection were achieved on an ACQUITY UPLC BEH-C18 column （2.1 mm×50 mm×1.7 μm） eluted with a gradient acetonitrile and 0.05 mol/L potassium dihydrogen phosphate solution at a flow rate of 0.2 mL/min， and detected at 350 nm. The results showed that there were good linear relationships （r=0.999 9） when the concentration of berberine hydrochloride and palmatine hydrochloride were in the range of 0.0372-0.2976 μg/mL and 0.0082-0.0656 μg/mL respectively， the recovery rates were more than 95%， and RSDs were less than 2%. This method was simple， rapid， accurate， and reproducible， and it can be used for determining the concentration of berberine hydrochloride and palmatine hydrochloride in Coptis.
Key words: Coptis； berberine hydrochloride； palmatine hydrochloride； UPLC；