陈  斌1， 俞凌云2， 徐亚欧1， 方  智2
（1. 西南民族大学生命科学与技术学院，四川 成都 610041； 2. 四川出入境检验检疫局检验检疫局技术中心，四川 成都 610041）
摘  要：为克服食品中复杂成分的干扰，提高稻瘟净的提取、净化效率及检测灵敏度，对已有稻瘟净的方法进行改进：用乙腈提取目标物稻瘟净，采用正己烷：丙酮（1∶1，V/V）替代传统工艺中毒性较强的二氯甲烷提取液；植物源性食品采用石墨化碳黑小柱对提取液进行净化，动物源性食品采用氧化铝柱对提取液进行净化；采用氮磷检测器（NPD）检测，方法检出限为0.001 mg/kg。测定食品中稻瘟净加标回收率在91.3%～97.5%之间，适应于成分复杂的食品中稻瘟净的测定。
关键词：稻瘟净；气相色谱-氮磷检测器；提取；净化
中图分类号：TS201.2；O657.7+1；TQ253.2+2；TS255.5     文献标志码：A    文章编号：1674-5124（2013）03-0045-03
Determination kitazin in foodstuffs by gas chromatography-nitrogen phosphorus detector
CHEN Bin1， YU Ling-yun2， XU Ya-ou1， FANG Zhi2
（1. Institute of Life Science and Technology，Southwest University for Nationalities，Chengdu 610041，China；
2. Technology Center of Sichuan Entry-Exit Inspection and Quarantine Bureau，Chengdu 610041，China）
Abstract: To overcome the interference of complex components of the foodstuffs， and improve the efficiency of extraction and purification of the kitazin， and detection sensitivity， the existing methods for determining kitazin in foodstuffs were effectively improved: acetonitrile was selected as extraction solution， and n-hexane: acetone （1∶1， V/V） was selected as the extraction solution， which were used to replace the toxic dichloromethane in traditional methods; graphitized carbon black small column were used to purify the extractant from plant foodstuffs， and alumina column were used for purifying extractant from animal foodstuffs. Nitrogen phosphorus detector （NPD） was chosen for detection with the detection limit of 0.001 mg/kg. The spiked recoveries of determination in foodstuffs were from 91.3% to 97.5%， and the method is suitable for determing kitazin in foodstuffs with complex matrix.
Key words: kitazin； GC-NPD； extraction； purification